Body temperature measurement in mice during acute illness: implantable temperature transponder versus surface infrared thermometry

Body temperature measurement in mice during acute illness: implantable temperature transponder versus surface infrared thermometry

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Abstract Body temperature is a valuable parameter in determining the wellbeing deus gorras of laboratory animals.However, using body temperature to refine humane endpoints during acute illness generally lacks comprehensiveness and exposes to inter-observer bias.Here we compared two methods to assess body temperature in mice, namely implanted radio frequency identification (RFID) temperature transponders (method 1) to non-contact infrared thermometry (method 2) in 435 mice for up to 7 days during normothermia and lipopolysaccharide (LPS) endotoxin-induced hypothermia.

There was excellent agreement between core and surface temperature as determined by method 1 and 2, respectively, whereas the intra- and inter-subject variation was higher for method 2.Nevertheless, using machine learning algorithms to determine temperature-based endpoints both methods had excellent accuracy in predicting death read more as an outcome event.Therefore, less expensive and cumbersome non-contact infrared thermometry can serve as a reliable alternative for implantable transponder-based systems for hypothermic responses, although requiring standardization between experimenters.